314 



REPRODUCTION 



The bulblets will be found to be extremely resistant to varia- 

 tions in temperature and moisture. 1 



404. Adventitious Buds of Asplenium bulbiferum. Observe a 

 number of specimens of Asplenium bulbiferum in a greenhouse 

 and note the numerous buds arising at various places on the 

 margins of the leaves. Select a portion entirely free from buds 

 and lay on moist sand to ascertain if the separated leaf is capable 



of giving rise to the buds or 

 new plants. Dissect a bud 

 and place the separated scales 

 in a proper culture chamber. 

 Are the leaves capable of re- 

 producing the plant? Test 

 the endurance of buds to 

 desiccation, and to tempera- 

 ture of dry and moist air. 



405. Adventitious Buds of 

 Polystichum annulare. Ex- 

 amine vigorous individuals of 

 Polystichum angulare, growing 

 in a green-house, for the ad- 

 ventitious buds which arise in 

 the axils of the pinnae. Dis- 

 sect and note structure. Ger- 

 minate in moist chamber, and 

 observe from what scales and 

 what regions the new shoots arise. Place the separate scales on 

 moist sand under a bell-glass and determine their capacity for pro- 

 pagation. Identify the storage material present. Place a portion 

 of the leaf in a moist chamber and find if it is capable of giving 

 rise to a new plant. Other species of Asplenium form similar 



1 Heinricher, E. Ueber die Wiederstandsfahigkeit der Adventivknospen von 

 Cystopteris bulbifera Bernh. gegen das Auftrocknen. Ber. Deut. Bot. Ges. 14 : 

 234. 1896. 



Heinricher, E. Nachtrage za meiner Studie ueber die Regenerationsfahigkeit 

 der Cystopteris Arten. Ber. Deut. Bot. Ges. 18: 109. 1900. 



Fig. 147. Brood-bodies of Lycopodium 

 lucidulum. After Atkinson. 



