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mycelium on seasoned wood was successful. The particulars of the work 

 so effected have appeared in the " Journal of the Forestry Society of 

 Japan " of April, 1904. 



II. Properties of the Shiitake Spore 



1. Spores germinate after a lapse of 24 hours in 16°C. or after 

 16 hours in 24°C. 



2. Between 95 and 96% of the spores generally germinate. 



3. Only 50-55% of spores kept for one month germinate. 



4. Only 2-3% of spores kept for 3 months germinate. 



5. Only 5-6% of spores kept after collection for 5 hours in sun- 

 light germinate. 



6. Only 1-5% of spores kept for 1 hour in 45°C. germinate. 



7. 30-35% of spores kept for 2 hours in 42°C. germinate. 



8. 80-90% of spores kept for 2 hours in ice germinate. 



9. 50-60% of spores kept in ice for 24 hours germinate. 



10. 10-15% of spores kept at 0°F. for 2 hours in a dried state 

 germinate. 



11. When spores in a nutrition liquid cooled at 0°F. for 1 hour 

 they lose their germinating power totally. 



12. The strong spores that retain germinating power in great heat 

 or cold thrive wonderfully. 



13. Spores germinate most readily in a decoction of Konara 

 (Quercus glandulifera Bl.) wood. 



III. Property of the Shiitake Mycelium 



1. Mycelium perishes when dried. 



2. Mycelium in the nutrition liquid, perishes when they are heated 

 at 40°C. 



3. Mycelium perishes when frozen. 



4. The growth of mycelium in a decoction of Konara is most 

 remarkable. 



5. In a decoction of mother logs thin mycelium is only slowly 

 produced. 



6. In starch or sugar solutions or in the mixture of both, 

 mycelium does not grow well. 



7. The same solutions acidified or alkalized show no difference. 



