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perish after standing 4 hours, nor do they perish in 24 hours at a 

 temperature below 0°C. 



Since the spores of this mushroom are not easily affected by the 

 cold, they can safely pass the winter in the woods. 



II, On the Mycelium of Matsudake 



1. The mycelium which germinated in distilled water, acid or alkaline 

 liquid grows rapidly at the ordinary temperature but they are not thick in 

 size. 



2. Mycelium grown in a decoction of pine roots makes rapid growth 

 and attains a length of 1 cm after 24 hours. 



3. The mycelium perishes immediately if dried. 



4. The mycelium does not freeze after 7 hours' standing, at 0°C. 

 The rapid growth of the mycelium from a spore will well explain 



how mycelium finds their hosts readily as soon as they fall on ground. 



III. How the Mushroom remains Parasite ? 



1. The pine mushroom is invariably found in Akamatsu woods 

 either alone or in association with other trees. Indeed, when the Akamatsu 

 is felled, the land will not produce a single mushroom after the year 

 immediately following. It should be noted that when the mushrooms are 

 removed from the original woods together with some of the surrounding 

 soil and kept carefully in pots, they will gradually fail to grow and the 

 next year no new mushroom will appear. This lead us to conclude that 

 the mushroom cannot live save as a parasite. 



2. Take the soil where the mushroom have just appeared for the 

 first time, say a pine forest of 15 years old and carefully study it around 

 the sprouts. Under the naked eyes, one notes that the upper layer of 

 the soil so taken is covered with raw humus and the rootlets which 

 have well developed coral rhizomes while the lower part of the soil 

 contains pine roots loaded with coral rhizomes and humus with clustered 

 white hyphae side by side with the ordinary mycelium from which the 

 mushroom grow. After careful study of one of these pine roots which 

 had mushrooms in the previous year, one notes that coatings of grey 



