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unchanged. This lead us to the final conclusion that the mushroom must 

 be taken as a true parasite thriving only on living trees. 



IV. Mycorhiza that takes the Pine Root as Host 



To successfully propagate Matsudake, one must commence with the 

 study of the host. To this end we have carefully examined the roots of 

 1-2 years old seedlings of Akamatsu (Pinus densifiora S. et Z.) and 

 Kuromatsu {Pinus Tunbergii Paris.) grown in the nurseries of the Station 

 and have noticed two distinct mycorhizas both leading a parasitic life on 

 the root. 



With the view of ascertaining whether the same mycorhiza are 

 invariably found in the root of pines of Japan or not, careful microscopical 

 studies have been made on Akamatsu and Kuromatsu seedlings grown in 

 the nursery of the Agricultural College of Komaba as well as on those 

 taken from the Domain Forest of Shirasu, Yamanashi Prefecture, and 

 from the Kyoto Local Nursery. The conclusion was that the mycorhizas 

 just referred to are the parasites common to seedling grown everywhere; 

 they invariably take these two pine species as hosts. 



1. EcTOTROPHic Mycorhiza of Akamatsu and Kuromatsu. 



It has long been known that European pines have ectotrophic my- 

 corhiza; we must, however, give thanks to Von Tubeuf for his discovery 

 that the Akamatsu is likewise affected with ectotrophic mycorhiza. 

 Whether this authority made the discovery on Akamatsu seedlings grown 

 in European soil or those imported from Japan is not stated ; we can not 

 identify the mycorhiza with that discovered by ourselves. At any rate, 

 we do not hesitate to say that the ectotrophic mycorhiza of the Akamatsu 

 grown in this country are composed of colorless mycelium of 2-3 micron 

 in diameter; they cover the outer parts of rootlets giving an appearance 

 of net work which sometime grow so heavy that the root may appear as 

 a white hairy substance to the naked eye. The reason why the parasite 

 may be classified as a mycorhiza lies in the fact that it possesses every 

 property of mycorhiza The same ectotrophic mycorhiza is, however, hardly 

 met with on the rootlets found in the raw humus which is particularly 

 suited to the growth of the mushroom. 



