— 119 — 



2. Endoteophic Mycorhiza of Akamatsu and Kuromatsu. 



The endotrophic mycorhiza found in Pinus sylvestris L. described 

 by MoUer do not seem to have been studied by the same author on 

 Japanese pines. After our close studies on the subject with regard to 

 Pinus densi flora S. et Z. and Pinus Thunbergii Pari, grown in Komaba 

 and Meguro nurseries, we succeeded in finding the said parasite. These 

 parasites are also found on the roots of the pine grown in localities where 

 the mushroom is known to grow well. 



V. The Mushroom Culture 



In the autumn of 1907, some 50 Akamatsu seedlings of 2 years old 

 were carefully up-rooted and washed so as not to injure the tender 

 rootlets. Every possible precaution was taken. The seedlings were put 

 in water and kept in a room where the temperature remained constantly 

 at 16°C. during the trial. The rootlets that had stopped their growth 

 for a time began to show activity with successive development of fresh 

 shoots. 



Later, the seedlings grown so promisingly in water were duly 

 inoculated with spores of mushrooms collected from the Crown Forest; 

 their daily progress was watched microscopically and it was learnt how 

 the mycelium coming out from spores succeed in finding their host in 

 the Akamatsu root. To our great regret, however, we could not continue 

 the researches on the plan above stated, since, as everyone is aware, the 

 seedlings of conifers in water culture are not fitted for long and delicate 

 experiments. The seedlings gradually began to fail; so, at last, we were 

 obliged to give up the original plan and substitute sand for water for 

 half of the surviving seedlings. The other half we planted in ordinary 

 pots with the necessary care and our pains were not entirely useless for 

 a small mushroom appeared in one of these pots during the autumn of 

 the next year, 1908. 



VI. Personal Researches in Mushroom Growing Districts 



Personal researches were made in places well known for mushroom 

 production, the result of investigations are briefly recorded herein. 



