94 UNIVERSITY OF WISCONSIN STUDIES 
Tue Liguerying CULTURES 
Fieure 1 shows that while there are liquefying cultures in 
all of the gas ratio groups, a large percentage of them form a 
mode which is quite distinct from that of the non-liquefying 
high ratio cultures. In other words, the cultures with a very 
high ratio are nearly all liquefiers. On account of the method 
of ealeulating percentages, the relative numbers of liquefying 
cultures are exaggerated. The ratio of approximately 1.06 was 
given by only three hquefying cultures, a number so small 
that they have not been considered at this time. 
502-00 £92 » 1§~30 
100_80 60 40 20 +|~ 20 40 60 80 /00 100 80 60 40 20 +|- 20 40 60 80 K 
T 
Indol. 
Saccharose| 
Roffinose. |___ oF mse 
Lactose, 
Starch, 
Inulin, 
Mannitol. 
Glycerol. 
Saheim, Laila) wef 
Dute:tol, 
Adonitol. | By Peres ee 
Fig. 5 
An additional mode over the infinity ratio marks a phys- 
iclogically distinet group. A comparison of Fig. 5 with Fig. 
2 shows that in fermentation reactions, the high ratio liquefy- 
ine cultures do not differ in a very marked degree from the 
high ratio non-liquefiers. Indol, saccharose, raffinose, inulin, 
elycerol, salicin and dulcitol are fermented in about the same 
way by the two groups. Starch is fermented more actively 
hy the non-liquefiers, and mannitol by the liquefiers. Tf, how- 
ever, we necept the arrangement shown in Fig. + we find that 
the liquefiers do not agree with either the adonitol + or adoni- 
tol — types. The liquefiers differ decidedly from the adonitol 
+ type in indol formation, and in the fermentation of adoni- 
tol and stareh, and from the adomtol— type in the fermenta- 
