124 UNIVERSITY OF WISCONSIN STUDIES 
trichum beurmanni from Gougerot of Paris, a sporotrichum 
isolated by K. F. Meyer from a horse in the United States, and 
a strain isolated in 1909 by the writer from typical sporotri- 
chosis in a man from North Dakota. The sera of the various 
rabbits were tested with the homologous organisms and also 
with other strains. It was found that agglutination appeared 
quite uniformily in the dilutions of serum varying from 
about 1/320 to 1/640. In most instances a slightly higher 
agglutination appeared in the homologous ‘sera but this was 
not always the case. The several strains tested could not be 
differentiated by these interagglutination tests in animals. 
Similar tests were made with serum from a human case in 
which agglutination with the homologous organism occurred 
at 1/160. Here again the original Sp. schenckii and Sp. 
beurmanne were agglutinated at approximately the same dilu- 
tion, namely, 1/160. The controls were negative. 
Wilder and McCullough” studied the serum from a case of 
sporotrichosis of the eye. Tests for agglutinins and opsonins 
in the serum of the patient against several strains of sporo- 
tricha, including the infecting strain, the original Schenck- 
Hektoen strain, a French strain, an equine strain from Mever, 
and two other American strains from typical cases, revealed 
no specific differences in the antibody content of the serum. 
The reaction of complement fixation is positive in cases of 
sporotrichosis but it seems less reliable than that of agglutina- 
tion. The studies of Widal and Abrami and other French 
workers have shown that the results are very definite but that 
an infection with many other mycoses (actinomycosis, hemis- 
porosis, discomycosis, ete.) will also give a positive test. A 
priort, then, one would not expect this test to be useful in 
differentiating strains of sporotricha. J. J. Moore*®® in our 
laboratory has made such studies, finding a definite fixation 
in human serum from a case of sporotrichosis using the homol- 
ogous organism. He obtained similar results when antigens 
made from the various other strains, including Sporotrichum 
schencku, Sporotrichum beurmanni and an equine strain, were 
27. A. M. A., 62, p. 1156, 1914. 
% Jour. Inf. Dis., 28, p. 252, 1918. 
