Case VIII.— (Animal No. 93.) Gelding, rising 3 years old, also showing symp- 

 toms of loco-poisoning as a yearling. On October 27, 1907, the condition was very 

 fair, symptoms only slightly noticeable; but during the past five months the disease 

 has greatly advanced until the present condition is quite as bad as the preceding 

 case. A fragment of the flexor metacarpi externus muscle only has been examined. 

 Sarcocysts were present, few in number, and in a young stage of growth. Previous 

 to lie finding of Sarcosporidiae in any of the foregoing cases, five loco-diseased equines 

 had succumbed. The parasites not being suspected none of the skeletal muscles were 

 miscroscopically examined. A portion of the myocardium was preserved in each case, 

 in which no parasites were detected. A section of the tongue of one of these animals 

 had been preserved, and in this Sarcosporidian cysts were found. 



(c) In Dourine-affected Equines. 



Case IX.— (Filly, 9 months *old.) Experimentally infected with Trypanosoma 

 Equiperdum. In the fluid of a cutaneous swelling that followed the inoculations free 

 spores of Sarcoysti together with trypanosomata were found, each species of para- 

 site being present in about equal numbers. 



Case X.— (Filly, 2 years old.) Inoculated with the blood of a Dourined stallion. 

 Seven months later, in the fluid of a cutaneous swelling, free Sarcospores were present, 

 mostly crescentic forms, and indistinguishable from the crescents described by certain 

 authors (see Plate II.) as developmental forms of Trypanosomata. Trypanosomes 

 were not seen in this animal at this time. 



Case XL— (Mare, 3 years old), in final stages of naturally acquired Dourine. 

 Sarcospores were found free in the fluid of a swelling on two occasions, the swellings 

 resulting from some serum-injection experiments. 



(d) In a Cachetic Filly, the Cause of the Cachexia Not Known. 



Case XII.— (Filly, 2 year's old), one of six supposedly healthy fillies shipped to 

 quarantine station for experimental work. It was noted on arrival, that this animal 

 (No. 68) had an unthrifty appearance, stiffened gait, rough hide and was in poor 

 flesh. The mucous membranes were pale. She was several times carefully examined 

 for signs of Dourine, but none were detected. The cachexia became more evident 

 during the autumn and winter, with increasing stiffness of the muscle and gait, especi- 

 ally of the hinder extremities. The animal was found dead in the pasture on March 

 4th last, the body frozen. 



The muscles of the oesophagus, tongue and extensors of the fore-arm were 

 infested with Sarcosporidiae. They were not seen in the myocardium or the muscle of 

 the eye; none other were examined. 



In cases I. to VIII. of Sarcosporidiosis in ' locoed ' animals certain symptoms 

 and conditions are described. These are more or less characteristic of ' loco-disease.' 

 This disease itself is now under investgation at this station and will be the subject 

 of a separate and later report, and is only mentioned here in those respects in which 

 it may bear a possible relation to Sarcosporidiosis, or where the writer is unable to 

 differentiate between the symptoms of the one '■and the other. Certainly, a great 

 variety of conditions and symptoms occur in the course of the malady or maladies 

 attributed to loco-poisoning. There appear various forms of mental derangement, 

 from mania in the first stages to a dense stupor or comatose condition in the latter ; 

 disorders of the muscular 'system from increased reflexes to spasms, atrophy and 

 paralysis; osseous enlargement or degeneration, especially of the jaws and parts of 

 the skull; impaired vision; depravity, emaciation, &c. The history in most cases 

 shows that the disease is very chronic, and often of a fluctuating or intermittent 

 character. The muscles of the head and neck, especially of the jaws, the lips, and the 

 tongue exhibit tremors, spasmodic contractions or prolonged after-contractions. The 



