12 



Fig. 4. Nos. 1-6. — Constriction of the protoplasm, and extended bodies (?), No. 4, 

 Suggesting that the pointed extremity of the cresent spore had penetrated one of 

 these bodies. 



Fig. 1-4. — Are taken from Sarcosporidiae of the ox. 



Fig. 5. — Represents types of spores found free in the fluid of cutaneous swellings 

 in dourine-affected equines. 



All figures in Plate II. are stained by the writer's modification of the Roman- 

 owsky method, prepared as follows: — A polychrome blue is made by one per eent 

 solution of medicinal methylene blue in a one-half per cent aqueous solution of 

 sodium bicarbonate, heating and evaporating to dryness over a water bath. The 

 residue is powdered and two solutions then prepared — 



A. — 35 com. B. 4 ccm. in 16 ccm. of methyl alcohol. 



B. 0'5 per cent eosin'in methyl alcohol and mixed in the following propor- 

 tions : — 



A.— 3'5 ccm. B. 4 ccm. in 16 ccm. of methyl alcohol. 



This solution is slightly alkaline (very useful for some purposes) for the study 

 of Hsematozoa and Sarcosporidiae. A drop or two of an exceedingly dilute solution 

 of acetic acid in alcohol should be added, care being taken not to over neutralize, and 

 the stain applied as in Leishmann's and other alcoholic Bomanowsky stains. 



The proportions of A and B may be varied slightly increasing the amount of 

 Eosin according to the reaction desired, and the stain may also be further diluted 

 with methyl alcohol to advantage. 



References. 



1. Numann, L. G. Parasites and parasitic diseases of domesticated animals. 



Second edition, Macqueen p. 555-561 — 1905. 



2. Minchin, E. A. — Sporozoa. — Treatise on zoology. Edited by Ray Lankester. 



Part 1—1903. 



3. Bulloch, W. — Psorospermosis. System of Medicine. Edited by Allbutt and 



Rolleston, Vol. II., Part II, p. 828—1907. 



4. Ostertag, R. — Handbook of Meat Inspection, translated by E. V. Wilcox. 



Second edition — 1905. 



5. Holmes, J. D. E. — Trypanosomiasis among cattle in India. Jour. Oomp. Path. 



and Therap. Vol. XVII., p. 323—1904. 



6. Lingard, A. — Trypanosomata observed in Bovines in India. Jour. Tropical 



Vet. Science, Vol. II., No. 1, p. 43—1907. 



7. Martin, E. E. — Trypanosomiasis in cattle of India. Jour. Comp. Path, and 



Therap. Vol. XVIIL, p. 145—1905. 



