392 Veterinary Medicine. 



Yz gallon, and water 20 gallons, (Th. Smith). The agents are 

 mixed slowly in a vessel set in cold water. The same may be 

 liberally applied on the surface, fences, etc., of the yards. Or 

 quicklime, freshly burned, may be used freely on the yards hold- 

 ing the infected flocks (Moore). Mercuric chloride is dangerous. 

 When the infected flock has been finally disposed of, the build- 

 ings and yards should be again thoroughly disinfected, and 

 together with the field runs, abandoned for at least one year. 



ASTHENIA IN CHICKENS. 



Microbiology : bacillus of colon group. Pathogenesis to Guinea pigs : 

 rabbits, pigeons and chickens refractory to artificial cultures. Treatment. 



Synonym. "Going I,ight," Bacterial Infection of the Duo- 

 denum. 



C. F. Dawson, in investigating a wasting disease of well fed 

 Brahma fowls in Maryland in which there was no appreciable 

 symptom except the gradual and extreme loss of flesh and weight 

 found in all cases a catarrhal duodenitis, with the presence in the 

 contents of a bacterium in pure cultures, and apparently allied to 

 bacillus coli communis. 



Bacteriology. The bacterium is i to 1.3 /a long, by .5 /x wide, 

 with rounded ends, often in pairs. It does not stain in acid nor 

 alkaline methylene blue, carbol fuchsin, nor alcoholic dyes, but 

 stains easily in aqueous solutions of the same stains and by 

 Gram's method. It is aerobic, facultative anaerobic, grows at 

 50° to 120° F. in acid or alkaline beef bouillon, with foetor ; in 

 glucose, saccharose, or lactose bouillon with the production of 

 acid ; in milk causing coagulation ; on gelatine, agar and potato. 

 Growth in Bouillon ceased at 131'' F. and sterilization took place 

 at 135° to 140° F. Vitality was not lost under freezing nor 

 drying. It was killed by a i per cent, solution of carbolic acid in 

 five minutes, or by formaldehyde gas, but not by lime water. 



Pathogenesis. Inoculated subcutem, in a Guinea pig caused 

 death in 24 hours, with a necrotic cedematous condition of the 

 adjacent tissues like malignant oedema, and containing the 

 microbe. It was further found in the liver, spleen, abdominal 

 exudate, heart, blood and lungs, but not in the kidneys. 



