462 Veterinary Medicine. 



1. Inject the herd at 10 or 11 p. m. to secure a good rest and 

 be fresh for the rise of temperature early next morning. 



2. Before injecting have the subjects arranged in order and 

 record them by name or other means of identification, with age, 

 sex, breed, weight, pregnant or not, past or prospective date of 

 calving, abortion, indications of disease, temperature taken just 

 before injection and appropriate dose. 



3. Inject into the loose connective tissue on the side of the 

 neck, the animal being held by the nose, if necessary, by an 

 assistant, 



4. Use a syringe which has not been employed for any infec- 

 tious products, and see that it is thoroughly cleansed and disin- 

 fected by boiling or by filling it with a carbolic acid solution 

 (5:100). 



5. After drawing the appropriate dose into the syringe, wipe 

 the nozzle and dip it in strong carbolic acid before inserting it 

 into the skin. This safely disinfects any virulent matter that 

 may be lodged on the surface of the skin, and obviates those in- 

 fected swellings and abscesses that have been a cause of com- 

 plaint by stock owners. 



6. When the nozzle is withdrawn from the skin, wipe it and 

 dip it again into the strong carbolic acid to prevent any risk of in- 

 fecting the tuberculin into which it is to be plunged. 



7. The nozzle is much more easily inserted in the skin if the 

 latter is pinched up so that the needle will transfix it at a right 

 angle, instead of passing through a greater amount of the dense 

 tissue because of the oblique direction. An excited animal with 

 a thick, tense hide and a contracted panniculus muscle will offer 

 serious obstruction which les-sens greatly as the subject gets over 

 its excitement and the muscle relaxes. 



8. Temperature .should betaken at 6 or 7 a. m., eight hours 

 after injection of tuberculin, and every two hours thereafter, 

 until the sixteenth hour. 



9. If any subject shows no rise of temperature until the i6th 

 hour after injection, its examination may be discontinued, but if 

 it shows a slight rise toward the i6th hour it should be continued 

 until it has shown a distinct reaction with steady rise and fall, or 

 until, without such distinct reaction, the temperature descends to 

 the normal. 



