Trembling in Sheep, etc. 503 



treated sheep sickened and died with lesions of louping-ill. On 

 the twenty-second day one of the muzzled sheep sickened, and 

 died of louping-ill next morning. On the thirty-first day another 

 of the muzzled sheep took ill and on the thirty-eighth was killed. 

 It showed characteristic symptoms and lesions. The seven salved 

 sheep, on which no ticks could at any time be found, remained 

 healthy throughout. 



Bacteriology. W. Williams describes a bacillus which he 

 figures as forming filaments of very uneven breadth, with fre- 

 quent branching (contrary to the habit of bacilli), and forming 

 spores in clusters. These were obtained from the coaguluni of 

 the cerebro-spinal fluid and Meek and Greig-Smith conclude that 

 the alleged mycelium was but the filaments of fibrin. 



McFadyean found pus microbes. 



Meek and Greig-Smith (Veterinarian, 1896-7) found in the 

 black bloody swellings under the skin, where the ticks had 

 inserted their proboscides, a variety of microbes which in pure 

 cultures did not provoke louping-ill. These included .staphylo- 

 coccus cereus albus, sarcina lutea, bacterium putridum, bacterium 

 coli commune, micrococcus sulphureus, micrococcus bicolor, and 

 micrococcus candicans. He also found penicilHum glaucum. 

 Two organisms allied to the bacterium fluorescens and designated 

 as j8 and 7 (G) respectively, were found in these .sores and pro- 

 duced in rabbits and sheep nervous disorders and lesions which 

 could be fairly identified with louping-ill. These are about 1.5 

 to 1.7/". long by 0.7/". broad, and chromogenic with a special fluor- 

 escent appearance. Microbe /8 inoculated on a rabbit caused on 

 the second day rhythmic movements of the head downward and 

 to one side, the eyelids closing as the head dropped, as if the 

 animal were constantly falling asleep. In another rabbit it caused 

 stiffness of the legs only and in a third it had no effect. Microbe 

 y when cultivated serobically was harmless to rabbits, but when 

 grown anaerobically on mutton bouillon to which a drop of blood 

 had been added, it caused on the second day general paralysis of 

 the neck and limbs, spasmodic twitching of the muscle.';, dyspnoea 

 and feeble heart action. Two rabbits which survived the early 

 effects developed large axillary and inguinal abscesses four 

 mouths later. 



A lamb inoculated with the bouillon culture of y showed after 

 thirteen days, lameness of one hind limb, with paresis, and a dis- 



