SYMPTOMS AND PATHOLOGY 



all Others, whose number is considerable, I obtained, 

 by the culture test in nutrient gelatine, evidence of the 

 presence in the lungs and the liver of numbers of 

 bacteria belonging to one and the same species ; for 

 when a particle of the lung or liver tissue is rubbed over 

 the slanting surface of nutrient gelatine, or when it is 

 shaken up in a test-tube with melted sterile nutrient 

 gelatine, and the latter is then poured into a sterile 

 glass dish, allowed to set and then incubated — that is 

 if a plate cultivation in gelatine is made — colonies of 

 one single bacterial species are obtained. By making 

 sections through the inflamed lung or liver, after har- 

 dening, and staining these sections for several hours 

 in methyl blue, or better still in rubin and methyl 

 blue, it is found that in some parts some of the 

 capillary blood-vessels, both in the lung and in the 

 liver, contain continuous masses, plugs or emboli, of 

 the same bacterial species (Figs. 2, 3, 4, 15 and i6).i 

 But there are seen under the microscope extensive 

 parts in which the blood-vessels do not contain them. 

 It is from this easy to see why I failed in 1887 to 

 obtain the microbe from the lung or liver in cover- 

 glass specimens or in culture, viz. I used in both 

 cases a droplet of the blood of the lung or liver, 



1 In sections through the hardened liver stained with rubin, then 

 with blue, the clumps of microbes are brought out with great dis- 

 tinctness as blue masses on a red ground. 



