CHAPTER IV 



EXPERIMENTS WITH CULTURE BY SUBCUTANEOUS 

 INOCULATION 



Experiments were made on tame mice with cultiva- 

 tions, — either with a particle of a gelatine- or potato- 

 or Agar culture distributed in, or mixed with, sterile 

 salt solution, the mixture being slightly turbid ; or 

 with a broth culture 24-48 hours to 3 days old. Of 

 the salt mixture or of the broth culture a few drops 

 (3-5 minims) are injected under the skin of the back. 

 The following day the animals are found quiet, and 

 they do not feed well ; the eyes are almost closed, the 

 back is curved, the coat rough, they look " lumpy " ; 

 the breathing is rapid, and, while motility becomes 

 weaker and weaker, they die either before 36 hours 

 are over or during the following night, i.e. before 

 48 hours. If the inoculation is made with a broth 

 culture 24-48 hours old, most of the mice inoculated 

 are dead before the end of the second day ; some 

 live till the end of the third day ; a few remain quiet 

 and ill and gradually recover. In the latter cases a 



