IV CULTURE BY SUBCUTANEOUS INOCULATION 39 



The most striking results were obtained on the 

 common bunting and yellow-ammer (greenback, cock- 

 bit, and finch), for the injection of a small drop of a 

 broth culture into the thigh is followed by fatal results. 

 The injected leg is swollen before the end of twelve 

 hours, the birds do not move that leg, and sit perched 

 on the other leg. Towards the end of the first day 

 they are quiet, "lumpy," their feathers are ruffled, 

 their breathing is very rapid, their eyes are closed ; 

 the animals are drowsy and have diarrhoea. Most of 

 them die towards the end of the first day, about the 

 20th-22nd hour; some die at about the 24th hour; 

 a few survive longer — even to the fourth day. On 

 post-mortem examination haemorrhagic infiltration is 

 found in the muscles of the thigh at the seat 

 of the inoculation, the lungs are deeply con- 

 gested, the liver is congested,' and so is the mucous 

 membrane and the serous covering of the intestine. 

 The spleen is dark and small. There is then a com- 

 plete analogy in these respects with the appearances 

 found in the grouse. The blood of the heart and the 

 sanguineous juice of the congested lungs teem with the 

 microbes (Figs. 18 and 19), either as single or dumb- 

 bell spherical and oval forms, or as single or dumb-bell 

 rods with rounded ends. Many of the white blood 

 cells in the lung juice contain in their substance the 

 bacilli : in some the bacilli are few in numbers, in 



