40 THE GROUSE DISEASE chap. 



Others the cell substance is crowded with them (Fig. 

 20). ■ Examined in the fresh state, some of the bacilli 

 show mobility. In dried and stained cover-glass speci- 

 mens and in cultivations from the heart's blood or 

 lung juice ; in plates, gelatine streak and stab, in 

 Agar, on potato, and in broth, the microbe shows 

 the same characters as that of the mouse (Fig. 21). 



The buntings and ammers possess even greater 

 susceptibility to the disease than the mice ; this is 

 shown by the fact that gelatine and Agar cultures, 

 which through age have become attenuated for mice 

 and guinea-pigs, still produce fatal results in these 

 birds. 



Sections made through the lung show many 

 capillaries and small vascular branches almost com- 

 pletely blocked by continuous masses of the microbe. 

 Staining the sections in rubin (2 per cent) for two 

 or three hours, then washing in water, then staining 

 in methyl blue aniline water for seven or eight 

 minutes, washing, dehydrating, passing through xylol, 

 and mounting in Canada balsam solution, shows the 

 cell nuclei and the bacilli blue, the rest of the 

 tissue red. 



Sparrows were also inoculated subcutaneously. 

 The great majority (70 per cent) die under the same 

 symptoms and with the same post-mortem appearances 

 as the ammers ; the microbes are present in the lungs 



