IV CULTURE BY SUBCUTANEOUS INOCULATION 43 



were found ill the day following the death of bird 

 No. I, and died in the course of the third day with 

 the symptoms and appearances of the disease ; in 

 their blood and lung the microbe was present in great 

 numbers. In a second set two wire cages were placed 

 close side by side ; one contained an ammer that was 

 affected with the disease after inoculation ; this bird 

 had been inoculated with attenuated culture (see 

 below), and remained in the diseased condition for 

 several days ; in the adjoining cage were six normal 

 ammers. The two cages were covered with one 

 cloth. All the six neighbours became ill with the 

 disease after two days, and died with the typical 

 symptoms and appearances during the following day. 



