CHAP, xii EXPERIMENTS ON FO WLS loi 



cultivations derived from the blood of the Orpington 

 fowls. From a broth culture (first sub-culture), after 

 twenty-four hours' incubation, about lO minims were 

 inoculated subcutaneously into each of the two fowls. 

 On the 6th day both animals were found quiet and dull, 

 off their food, and suffering from diarrhoea. On the 

 7th day both were found dead with the characteristic 

 post-mortem appearances ; cover-glass specimens and 

 cultivations of the heart's blood, spleen tissue, and 

 intestinal mucus yielded abundant evidence of the 

 presence of the bacilli. 



4. From a second broth sub-culture twenty -four 

 hours old, which had been established from a previous 

 gelatine sub-culture, two fowls were inoculated. Both 

 showed the first signs of illness on the 6th day, being 

 quiet, off their food, and affected with diarrhoea. 

 One of these fowls was found dead on the 7th day, 

 with the typical appearances, and the bacilli in the 

 blood, spleen, and intestinal mucus. The . second 

 one was still quiet and had diarrhoea on the 8th 

 day. On the 9th day it still had diarrhoea, but 

 appeared a little more lively and was seen feeding. 

 On the lOth day the diarrhoea was less, and the fowl 

 was distinctly better. The diarrhoea gradually sub- 

 sided, and at about the 1 8th -20th day the fowl seemed 

 to have perfectly recovered. 



Two other fowls were inoculated subcutaneously 



