THE GROUSE DISEASE 



with several minims of a broth sub-culture, twenty-four 

 hoTjrs old. Both birds showed the first signs of illness 

 on the 5th day ; they were quiet, off their food, and 

 had diarrhoea. The condition was the same on the 

 6th and 7th days ; on the 8th day the diarrhoea was 

 less, the birds were livelier, moved about, and 

 fed also a little; they gradually recovered from the 

 diarrhoea, and by the end of the third week had 

 seemingly quite recovered. 



[I shall have occasion to refer further below to a 

 large number of inoculation experiments on fowls made 

 with broth cultures, but as they were made with broth 

 cultures previously exposed to certain higher degrees 

 of temperature, with the object of attenuation, they will 

 be described in connection with the protective inocula- 

 tions.] 



In order to see whether the infection of fowls can 

 be produced by feeding, the following experiments 

 were instituted : 



5. Some of the intestinal contents of a fowl that 

 had died of the disease after inoculation with blood 

 and spleen tissue of an Orpington fowl, were poured 

 down the throat of two fowls. One of these birds 

 showed the first signs of illness — viz. slight diarrhoea 

 on the 6th day, oii the 7th day the diarrhoea was pro- 

 fuse, the bird was quiet, dull, and off its food. This 

 animal was found dead on the 8th day. On post- 



