"CRAMPS" IN YOUNG PHEASANTS 127 



ferred to test-tubes containing nutrient gelatine and 

 nutrient Agar set with slanting surface, and is well 

 rubbed over the slanting surface. The gelatine tubes 

 are incubated at 20° C, the Agar tubes at 36-37° C. 

 All the tubes thus inoculated developed several 

 colonies of one and the same species of bacilli. In 

 the gelatine tube I noticed after 2-3 days the first 

 indications of the colonies in the shape of minute 

 translucent greyish droplets; in Agar the colonies 

 are noticeable after one day's incubation. The 

 colonies, even when well developed, are from the size 

 of a pin's point to that of a millet seed. Streak sub- 

 cultures on gelatine are very characteristic, this being 

 due to two facts — {a) the slowness of the growth, the 

 line of inoculation becoming marked as a line of small 

 greyish transparent droplets, not before two to three 

 days ; [b) as the droplets enlarge they have a tendency 

 to become more or less confluent, but they always 

 remain recognisable as individuals — there is not a con- 

 tinuous band-like growth produced, as in most streak 

 cultures of bacilli. The same applies to Agar streak 

 cultures, only of course the growth is faster, but also 

 here the individuality of the colonies remains more or 

 less preserved even where the colonies are situated 

 closely and uninterruptedly side by side. In old 

 gelatine- and in old Agar cultures the colonies be- 

 come whitish, raised, knob -like, their outline slightly 



