222 PARASITOLOGY. 
oil of cloves. The first clearing agent will perhaps 
give the best results, or as soon as the worms are col- 
lected and washed, dropped in an 80% alcohol at 80°. 
This causes the worms to straighten out and kills them 
at once. They are then placed in 80% alcohol to which 
10% glycerin has been added then 20% glycerin grad- 
ually increasing to full strength till the bodies are 
clarified. The internal anatomy can now be studied. 
The parasites may be stained with alcoholic eosin and 
clarified in beechwood creosote and kept in small bot- 
tles in creosote or mounted in Canada balsam. Care 
must be exercised in placing them in the glycerine to 
not let them stay too long lest they become too trans- 
parent. 
PRESERVATION OF MUSEUM SPECIMENS. 
The Keiserling method gives the best results be- 
cause the tissues retain their normal color. This 
methodconsists of three steps as follows: 
1. Place in the following solution and leave from 
one to seven days, depending upon the size of the 
specimen : 
Formalin.__-.-_-__----- 200cc 
Potassium Acetate_____ 30 grams 
Potassium Nitrate_____ 15 grams 
Water oot wean ccce 1000 cc 
2. Pass the specimen through each of the follow: 
ing solutions, leaving it in each for twenty-four 
hours. This should restore the normal color: 
40% Alcohol © 
60% Alcohol 
80% Alcohol 
95% Alcohol 
