H^MANTHUS. 



49 



Gentian violet: 



H. katherinse, moderate to deep; value 60. 



H. punioeus, moderately deep to deep, slightly deeper than H. 



katherinse; value 62. 

 H. konig albert, moderate to deep, not so deep as in the parents, 



value 58. 

 Safranin: 

 H. katherinse, moderate to deep; value 60. 

 H. punioeus, moderately deep to deep, slightly deeper than in H. 



katherinse, value 62. 

 H. konig albert, moderate to deep, not so deep as in the parents, 



value 58. 

 Temperature: 



H. katherinse, majority at 79 to 80°, all at 82 to 84°, mean 83°. 

 H. punioeus, majority at 77 to 79°, all at 81 to 82.5°, mean 81.75°. 

 H. konig albert, majority at 80 to 82°, all at 82.5 to 84°, mean 83.25°. 



The reactivity of H. Icatherinw is higher than that 

 of the other parent in the reaction with iodine and lower 

 in those with polarization, gentian violet, safranin, 

 and temperature. The hybrid is mid-intermediate in 

 the iodine reaction, the highest in the polarization reac- 

 tion, lowest in the gentian violet and safranin reactions, 

 and the same as that of the seed parent in the tempera- 

 ture reaction. In three it is closer to or the same as 

 the seed parent, in one closer to the pollen parent, and 

 in one mid-intermediate. 



Table A 6 shows the reaction-intensities in percent- 

 ages of total starch gelatinized at definite intervals 

 (minutes). 



Vblocity-eeaction Curves. 



The following section deals with velocity-reaction 

 curves of the starches of Hcsmanthus Icatherinw, H. pu- 

 niceus, and H. honig albert, showing the quantitative 

 differences in the behavior toward different reagents 

 at definite time-intervals. (Charts D 106 to D 126.) 



The most conspicuous features of these charts are : 



( 1 ) The marked tendency for the curves of H. kath- 

 erincB and the hybrid to run together, usually very 

 closely, and well separated from the curve of H. pwniceus. 

 Both features are well exhibited in all of the reactions, 

 with the exception of those with chloral hydrate, pyro- 

 gallic acid, sodium salicylate, and barium chloride. 

 Even in these instances the closer relationship of H. 

 katherinw and the hybrid is evident. 



(2) The tendency for the curve of the hybrid to an 

 intermediate position between those of the parent-stocks, 

 although distinctly closer to that of H. hatherince, as 

 shown in the reactions with chromic acid, pyrogallic 

 acid, nitric acid, sulphuric acid, hydrochloric acid, and 

 sodium salicylate. In the chloral-hydrate reaction the 

 curve of the hybrid is curiously distinctly lower than 

 that of either parent. In the' remaining reactions, 14 

 in number, the starches of both H. hatherincB and the 

 hybrid are so resistant that such differences as are re- 

 corded are slight and fall within the limits of error. 

 From experience with other resistant starches modifi- 

 cations in the strengths of the reagents would doubtless 

 elicit peculiarities in accord with the foregoing. 



(3) The individuality of each of the charts with few 

 exceptions ; hence, the peculiarity of each chart in specific 

 relation to the reagent. Some bear somewhat close 

 resemblances, as for instance, those particularly of 

 pyrogallic and nitric acid, and those of another group 

 including the potassium iodide, potassium hydroxide, 

 potassium sulphocyanate, potassium sulphide, sodium hy- 

 droxide, sodium sulphide, calcium nitrate, strontium 

 nitrate, and cupric chloride, in which the main differ- 

 ence between the positions of the curves lies in the height 

 of the curves of Et. puniceus. The curves of the sodium- 

 salicylate reactions are of a markedly different character 

 from those of other chemical reagents because of the 

 high reactivities of all three starches. High reactivities 

 of H. puniceus are also exhibited in the charts for pyro- 



Table a 6. 



Chloral hydrate: 



H katherinse 



H. punioeus 



H. konig albert 



Chromic acid: 



H. katherinse 



H. punioeus 



H. konig albert 



Pyrogallic acid : 



H. katherinse 



H. punioeus 



H. konig albert 



Nitric acid: 



H. katherinse 



H. puniceus 



H. kSnig albert 



Sulphuric acid: 



H. katherinse 



H. puniceus 



H. konig albert 



Hydrochloric acid: 



H. katherinse 



H. puniceus 



H. konig albert 



Potassium hydroxide: 



H. katherinse 



H. punioeus 



H. konig albert 



Potassium iodide: 



H. katherinse 



H. puniceus 



H. konig albert 



Potassium sulphocyanate : 



H. katherinse 



H. puniceus 



H. konig albert 



Potassium sulphide: 



H. katherinse 



H. puniceus 



H. konig albert 



Sodium hydroxide : 



H. katherinse 



H. puniceus 



H. konig albert 



Sodium sulphide: 



H. katherinse 



H. puniceus 



H. konig albert 



Sodium salicylate: 



H. katherinse 



H. punioeus 



H. konig albert 



Calcium nitrate: 



H. katherinse 



H. punioeus 



H. konig albert 



Uranium nitrate: 



H. katherinse 



H. punioeus 



H. konig albert 



Strontium nitrate: 



H. katherinse 



H. punioeus 



H. konig albert 



Cobalt nitrate : 



H. katherinse 



H. puniceus 



H. konig albert 



Copper nitrate: 



H. katherinse 



H. puniceus . . . . 



H. kiinig albert 



Cupric chloride: 



H. katherinse 



H. punioeus 



H. konig albert 



Barium chloride: 



H. katherinse 



H. punioeus 



H. konig albert 



Mercuric chloride: 



H. katherinse 



H. punioeus 



H. konig albert 



7 



14 



3 



1 



27 

 0.5 



3 



65 



2 



0.5 



60 







10 

 95 

 10 



1 



80 



1 



1 

 70 

 1.5 



1.5 



52 



1 



2.5 



72 

 1 



1 



45 



1 



1 



61 

 0.5 



0.5 



50 



2 



80 

 60 

 65 



1 

 50 

 0.5 



1 

 26 

 0.5 



2 



44 

 0.5 



0.5 



4 



0.5 



0.5 

 11 

 0.5 



0.5 

 37 

 0.5 



1.25 

 1.5 

 0.5 



1.25 



7 



0.5 



56 



60 



67 



99 



97 



57 



30 



14 



53 



3.5 



73 



100 



60 



35 



10 



15 



3 4 

 90 95 

 11 14 



17 



80 



12 



16 



56 



20 



74 

 82 

 61 



97 

 99 

 98 



30 

 99 

 33 



6 

 96 

 22 



94 



99 



15 

 97 

 67 



2 



98 



4 



3 

 92 

 1.5 



4 



90 



4 



2 



70 

 2 



3 



84 

 2 



2 

 60 

 2.5 



1 

 62 

 0.5 



1.25 

 35 

 0.5 



3 



78 

 1 



1 

 14 

 0.5 



1.5 

 24 

 0.5 



0.5 

 59 

 0.5 



1.5 



6 



0.5 



1.5 

 22 

 0.5 



