64 



HISTOLOGIC PROPERTIES AND REACTIONS. 



major, and in the gentian-violet, safranin, and tempera- 

 ture reactions higher reactivities. Both hybrids in the 

 polarization and temperature reactions show higher reac- 

 tivities than either parent, both being in both reactions 

 closer to N. bowdeni than to the other parent, but in the 

 temperature reaction N. abundance is practically the 

 same as N. bowdeni. The hybrid N. giantess in the 

 iodine reactions is the same as N. sarniensis var. corusca 

 major, but N. abundance is the same as the other parents. 

 N. giantess is the same as N. bowdeni in the gentian- 

 violet reactions, vrhile N. abundance is the same as the 

 other parent. N. giantess is the same as N. bowdeni 

 in the safranin reactions, while N. abundance is inter- 

 mediate between the parents, but closer to N. bowdeni. 



Table A 11 shows the reaction-intensities in percent- 

 ages of total starch gelatinized at definite intervals 

 (minutes). 



Velocity-eeaction Curves. 



This section treats of the velocity-reaction curves of 

 the starches of Nerine bowdeni, N. sarniensis var. corusca 

 major, N. giantess, and N. abundance, showing the quan- 

 titative differences in the behavior toward different reag- 

 ents at definite time-intervals. (Charts D' 211 to D 231.) 



Among the most conspicuous features of these charts 

 are: 



(1) The marked closeness and correspondence in the 

 courses of all four curves, excepting in the reactions 

 with chloral hydrate and potassium sulphocyanate, as 

 was noted in the preceding set. Owing to too rapid, too 

 slow, or too close reactions no satisfactory if any differ- 

 entiation can be made in the reactions with pyrogaUic 

 acid, sulphuric acid, hydrochloric acid, potassium hy- 

 droxide, sodium sulphide, cobalt nitrate, cupric chloride, 

 barium chloride, and mercuric chloride. 



(2). The curve of N. bowdeni is higher than the curve 

 of the other parent in the reactions with chromic acid, 

 nitric acid, potassium iodide, potassium sulphocyanate, 

 sodium hydroxide, calcium nitrate, uranium nitrate, and 

 cupric chloride ; and lower in those with chloral hydrate, 

 potassium sulphide, sodium salicylate, and strontium 

 nitrate. 



(3) The curves of the hybrids bear varying relation- 

 ships to the parental curves, and the hybrid curves them- 

 selves differ in many respects from each other. There is 

 in N. giantess a distinct tendency to intermediateness 

 and to the lowest position in relation to the parental 

 curves, and with a decided inclination to the curves of 

 the pollen parent; while in N. abundance there is a 

 particularly marked inclination to be the highest of the 

 three curves and to the curves of the pollen parent. 



(4) An early period of high resistance followed by 

 a rapid to moderate gelatinization is noted in very few 

 of the experiments, but especially in the chromic-acid 

 reaction. 



(5) The earliest period during the 60 minutes that 

 is best for the differentiation of dl four starches is for 

 chloral hydrate, nitric acid, potassium sulphide, sodium 

 salicylate, and strontium nitrate at 5 minutes ; for potas- 

 sium iodide at 30 minutes ; for potassium sulphocyanate, 

 sodium hydroxide, calcium nitrate, uranium nitrate, and 

 cupric chloride at 60 minutes. Other reactions are too 

 slow or too fast for satisfactory differentiation. 



