80 



HISTOLOGIC PROPERTIES AND REACTIONS. 



Safranin: 



N. abscissus, moderate, value 47. 



N. poeticus poetar., moderate, somewhat more than in N. abscissus, 



value 50. 

 N. will scarlet, moderate, higher than in either parent, value 53. 

 Temperature : 



N. abacissus, in majority at 69.5 to 71°, in all at 73 to 74°, mean 



73.5°. 

 N. poeticus poetar., in majority at 69 to 71°, in all at 71 to 73°, 



mean 72°. 

 N. wiU scarlet, in majority at 69.8 to 71.9°, in all at 72 to 74°, 



mean 73°- 



The reactivity of N. abscissus i8 the same or practi- 

 cally the same as that of the other parent in not a single 

 reaction; higher in the polarization reaction; aoad lower 

 in those with iodine, gentian violet, safranin, and tem- 

 perature. The reactivity of the hybrid is the same or 

 practically the same as that of N. abscissus in the polar- 

 ization reaction; the same or practically the same as 

 that of the other parent in the iodine reaction ; and the 

 highest of the three in the reactions with gentian violet 

 and safranin; and intermediate but close to the seed 

 parent in the temperature reaction. 



Table A 18 shows the reaction-intensities in percent- 

 ages of total starch gelatinized at definite intervals 

 (minutes) : 



Table A 18. 



Velocity-eeaction Cueves. 

 This section treats of the velocity-reaction curves of 

 the starches of Narcissus abscissus, N. poeticus poetarwm, 

 and N. will scarlet, showing qualitative differences ia the 

 behavior toward different reagents at definite time- 

 intervals. (Charts D 305 to D 310.) 



The most conspicuous features of these charts are : 

 (1) The close correspondence of all three curves 

 (excepting in the pyrogallic-acid reaction, in which there 

 is a disproportionate separation of the curve of N. ab- 

 scissus from the other curves) ; and also the tendency 

 for the reactions, excepting that with sulphuric acid, to 

 be of moderate to low or very low intensity. The sul- 

 phuric-acid reaction is so very rapid that there is no 

 differentiation to be seen in the charts, although, as will 

 be seen from the preceding table, the reactivity of N. 

 poeticus poetanim is less at first than that of either of 

 the other starches. In the chloral-hydrate reaction the 



differences are of a very minor character, not sufficient 

 for satisfactory differentiation. 



(2) The relations of the parental curves to each 

 other and to the hybrid vary in the reactions, and in the 

 pyrogallic-acid reaction they vary during their course. 



(3) The curve of N. abscissus is higher than that of 

 the other parent in the reactions with chromic acid, pyro- 

 gallic acid, and nitric acid, in the two latter being quite 

 well separated. A higher reactivity of N. abscissus is 

 also indicated in the records of the reactions with chloral 

 hydrate and sulphuric acid. 



(4) The curve of the hybrid is the highest of the 

 three in the reactions with chromic acid and nitric acid, 

 and intermediate during the first part and lowest during 

 the latter part of that with pyrogallic acid, although in 

 this reaction there are but small differences between the 

 hybrid and N. poeticus poetarum. 



(5) An early period of resistance followed by com- 

 paratively rapid gelatinization is noted in all three 

 starches in the reaction with chromic acid, in two with 

 pyrogallic acid, and in one with nitric acid. The reac- 

 tion with sulphuric acid is too rapid and with chloral 

 hydrate too slow for a manifestation of this peculiarity. 



(6) The earliest period at which the curves are best 

 separated for differential purposes varies in the different 

 reactions. This period is approximately in the reactions 

 with sulphuric acid and pyrogallic acid within the 5-min- 

 ute interval; in those with chromic acid and pyrogallic 

 acid at the 15-minute interval ; and in the chloral-hydrate 

 reaction at probably 30 to 45 minutes, although at any 

 time the differences in this reaction may fall wholly 

 within the limits of error of experiment. 



Reaction-intensities oe the Hybrid. 



This section treats of the reaction-intensities of the 

 hybrid as regards sameness, intermediateness, excess, 

 and deficit in relation to the parents. (Table A 18 and 

 Charts D 305 to D 310.) 



The reactivities of the hybrid are the same as those 

 of the seed parent in the polarization and sulphuric acid ; 

 the same as those of the pollen parent in the iodine reac- 

 tion; the same as both parents in that with chloral hy- 

 drate ; intermediate in those with temperature and pyro- 

 gallic acid (in one being closer to one parent and in the 

 other closer to the other parent) ; highest in those with 

 gentian violet, safranin, chromic acid, and nitric acid 

 (in three being closer to the pollen parent, and in one 

 closer to the seed parent) ; and lowest in none. 



The following is a summary of the reaction-intensi- 

 ties (10 reactions) : Same as seed parent, 2; same as 

 pollen parent, 1 ; same as both parents, 1 ; intermediate, 

 2 ; highest, 4 ; lowest, 0. 



The seed parent has probably slightly more influence 

 than the pollen parent in determining the properties of 

 the hybrid. The tendency of the hybrid to highness is 

 evident, this being more marked than to intermediateness. 



Composite Cueves of the Reaction-intensities. 

 This section treats of the composite curves of the 



reaction-intensities, showing the differentiation of the 



starches of Narcissus abscissus, N. poeticus poetarum, 



and N. will scarlet. (Chart E 18.) 



The most conspicuous features of this chart are: 

 (1) The close correspondence of the three curves 



both as to cloeeness and course, the only tendency even 



