NARCISSUS. 



89 



deficit in relation to the parents. (Tables A 23 and 

 Charts D 335 to D 340.) 



The reactivities of the hybrid are the same as those 

 of the seed parent in the reactions with polarization, io- 

 dine, gentian violet, and sulphuric acid; the same as 

 those of the pollen parent in none; the same as those 

 of both parents in the safranin reaction; intermediate 

 in those with temperature, chloral hydrate, and pyro- 

 gallic acid, in two bedng closer to those of the pollen 

 parent and in one as close to one as the other parent: 

 highest in the nitric-acid reaction, and closer to the 

 pollen parent; and lowest in the chromic-acid reaction, 

 being closer to the seed parent. 



The following is a summary of the reaction-intensi- 

 ties (10 reactions) : Same as seed parent, 4; same as pol- 

 len parent, ; same as both parents, 1 ; intermediate, 3 ; 

 highest, 1 ; lowest, 1. 



From the foregoing data it seems that the seed 

 parent exercises a distinctly greater influence than the 

 pollen parent on the characters of the starch of the 

 hybrid. The most marked tendencies in the reactions 

 are to sameness as the seed parent and to interme- 

 diateness. 



Composite Curves op KEACTioN-iiirTENsiTiES. 



This section treats of the composite curves of the 

 reaction-intensities, shovnng the differentiation of the 

 starches of Narcissus leedsii minnie hume, N. triamr- 

 drus albus, and N. agnes harvey. (Chart E 33.) 



The most conspicuous features of this chart are : 



( 1 ) The very close correspondence of all three curves 

 in course and closeness throughout the chart. 



(2) In N. leedsii minnie hume in comparison with 

 the other parent the higher gentian-violet and chloral- 

 hydrate reactions ; the lower reactions with polarization, 

 iodine, temperature, chromic acid, pyxogallic, and nitric 

 acid; and the same or practically the same in the reac- 

 tions with safranin and sulphuric acid. 



(3) In iV. leedsii minnie hume the very high sul- 

 phuric-acid reaction ; the high iodine reaction ; the mod- 

 erate polarization and safranin reactions; the low reac- 

 tions with gentian violet, temperature, chromic acid, 

 pyrogallic acid, and nitric acid; the very low reaction 

 with chloral hydrate. 



(4) In N. triandrus albus the very high sulphuric- 

 acid reaction; the high iodine reaction; the moderate 

 reactions with polarization, safranin, chromic acid, and 

 pyrogallic acid; the low reactions with gentian violet, 

 temperature, and nitric acid; and the very low reaction 

 with chloral hydrate. 



(5) In the hybrid the very high sulphuric-acid reac- 

 tion; the high iodine reaction; the moderate polariza- 

 tion and safranin reactions ; the low gentian-violet, tem- 

 perature, chromic-acid, pyrogallic-acid, and nitric-acid 

 reactions ; and the very low chloral hydrate reaction. 



The following is a summary of the reaction-intensi- 

 ties (10 reactions) : 



24. COMPAEISONS OB" THE StAECHES OF NaECISSUS 

 EMPEEOE, 'N. TEIANDEUS ALBUS, AND N. J. T. 

 BENNETT POE. 



In histologic characteristics, polariscopic figures, 

 reactions with selenite, reactions with iodine and quali- 

 tative reactions with the various chemical reagente, the 

 starches of the parents and hybrid exhibit properties in 

 common in varying degrees of development which col- 

 lectively in case of each starch are distinctive. The 

 differences are of a minor character. In histologic prop- 

 erties in Narcissus triandrus albus in comparison with 

 the other parent there axe more compound grains and 

 aggregates, together with various other peculiarities, 

 and there are various other differences in hilum, lamellae, 

 and size. The polariscopic figure is not so distinct 

 but more often well defined, and there are other minor 

 differences. Wdth selenite the quadrants are more often 

 clean-cut, the colors less often pure, and fewer grains 

 with a greenish tinge. In the qualitative reactions with 

 iodine no distinctive differences were recorded. In the 

 qualitative reactions with chloral hydrate, chromic acid, 

 pyrogallic acid, nitric acid, and sulphuric acid both 

 methods of gelatinization common to both starches occur, 

 and also methods observed in N. triandrus albus that 

 are not seen or seen only in modified form in N. emperor. 

 The starch of the hybrid contains fewer compound 

 grains and aggregates than either parent, and shows, 

 on the whole, a closer relationship to N. emperor than 

 to the other parent. In character and eccentricity of 

 the hilum and in size the relationship is closer to N. 

 emperor; but in the character of the lamellae closer to 

 N. triandrus albus. In the character of the polariza- 

 tion figure and in the reactions with selenite the relation- 

 ship is closer to N. triandrus albus. In the qualitative 

 reactions with iodine the raw grains are more closely 

 related to those of N. emperor, but the gelatinized grains 

 show no differences from those of both parents. In the 

 qualitative reactions with the chemical reagents' the in- 

 fluences of both parents are manifest; in the chloral 

 hydrate and sulphuric acid the resemblances are closer 

 to N. emperor, while in the chromic acid, pyrogallic acid, 

 and nitric acid the hybrid is closer to N. triandrus albus. 



Reaction-intensities Expressed by Light, Color, and Tempera- 

 ture Reactions. 

 Polarization : 



N. emperor, low to high, value 60. 



N. triandrus albus, low to high, lower than in N. emperor, value 50. 

 N. j. t. bennett poe, low to high, the same as in N. triandrus albus, 

 value 60. 

 Iodine : 



N. emperor, moderate to deep, value 60. 



N. triandrus albua, moderately deep, deeper than in N. emperor, 



value 65. 

 N. j. t. bennett poe, moderate to deep, the same as in N. emperor, 

 value 60. 

 Gentian violet: 



N. emperor, moderate, value 45. 



N. triandrus albus, light to moderate, lighter than in N. emperor, 



value 35. 

 N. j. t. bennett poe, moderate, deeper than in either parent, value 50, 

 Safranin : 



N. emperor, moderate, value 60. 



N. triandrus albua, light to moderate, lighter than in N. emperor, 



value 40. 

 N. J. t. bennett poe, moderate, deeper than in either parent, value 55. 



