70 W. M. SMALLWOOD AND RUTH L. PHILLIPS 



questioned the results obtained by Hodge and Crile. Oiir yrork 

 was begun in 1910-11, but the opportunity for completing it 

 did not present itself until this summer. We have re-examined 

 our earher work and supplemented it with additional material 

 collected and prepared in the same way as that obtained 

 previously. 



This material consists of the following stages covering the life 

 cycle of the honey bee. 



1. Recently hatched larvae. 



2. Half-grown larvae. 



3. Fully-grown larvae. 



4. Early pupae. 



5. Mid-pupae. 



6. Late pupae. 



7. Newly hatched adults. 



8. Young adults taken at 6.30 a.m. 



9. Yoimg adults taken at 6.30 p.m. 



10. Old adults taken at 6.30 a.m. 



11. Old adults taken at 6.30 p.m. 



12. Adults taken at close of the winter season. 



Several different fixatives were tried, but the only ones found 

 successful were osmic sublimate, 1 per cent osmic acid, 1 per 

 cent glacial acetic, and sublimate to saturation, Camoy's and 

 Ohhnacher's fluids. Only one individual, that one of stage (8), 

 included in our study was fixed with osmic sublimate. 



No attempt was made to dissect out the brains of the larvae, 

 which were embedded entire. The brains of pupae and adults 

 were excised. Sections were cut from four to seven micra thick 

 in paraffin of 54°, and stained in iron haematoxylLa with Bordeaux 

 red as a counter stain. 



The Zeiss and Leitz eyepiece micrometers were used, readings 

 being computed in micra. We tried to use the planimeter in 

 our work this summer, but found it impracticable in measuring 

 such small nuclei. 



There are according to Kenyon,^ four general regions in the 

 brain of the bee; the dorso-cerebron, the ventro-cerebron, and 



' Journal of Comparative Neurology, vol. 6, 1896. 



