36 REPRODUCTION OF BACTERIA. 
III. Staining Spores. 
(1) First Method. 
(a) Prepare films in the usual manner. 
(6) Stain in warm Ziehl’s carbol-fuchsin ' for 
twenty to thirty minutes, or in the same solution 
steaming, but not boiling, for five minutes. 
(c) Wash in water. 
(a2) Dip in acid alcohol (70 per cent. alcohol, 97 
Fic. 18.—Bacterium anthracis, stained to show spores; 
x 1000 (Frankel and Pfeiffer). 
c.c. ; hydrochloric acid, 3 ¢.c.) or in I per cent. 
sulphuric acid a few seconds and wash in water. 
(e) Examine in water. 
Spores should be red and rods unstained or 
faintly eee If the spores are not sufficiently 
2 # See Appendix, page 172. 
