38 REPRODUCTION OF BACTERIA. 
(2) Spread a loopful on a cover-glass; dry; fix. 
(e) Decolorize in a 14 per cent. (by volume) solu- 
tion of hydrochloric acid in alcohol. 
Fic. 19.—Bacillus chauveei, showing spores ; x 1000 
(Frankel and Pfeiffer). 
(/) Wash in water and contrast-stain in Léffler’s 
methylene-blue. 
(g) Wash, dry, and mount. 
(4) To Observe the Germination of Spores. 
(a) Make a hanging-drop preparation, using ster- 
ile bouillon and some of the spores from the above 
culture. 
(4) Place on a warm stage and watch the growth 
of rods from the spores. 
(c) Inoculate a sterile bouillon-tube with a large 
number of spores from the above culture. 
(2) Make stained cover-glass preparations from 
this at intervals of one-half hour. 
