BOUILLON—GELATIN—AGAR. 53 
17. Tube and sterilize for fifteen minutes in the 
steam sterilizer on three successive days. 
Some of the gelatin- and agar-tubes after the 
Fic. 28.—Funnel for filling tubes with culture-media : 
a, funnel containing the culture-media in liquid condition ; 
6, pinch-cock by which the flow of fluid into the test-tube 
is regulated ; c, rubber tubing (Warren). 
1 Allow the chamber to fill with steam before placing the 
media within. Do not leave the media in the sterilizer to 
cool. Plunge gelatin-tubes into ice-water immediately after 
each sterilization, in order to maintain a high melting-point. 
