56 PREPARATION OF CULTURE-MEDIA. 
the steam sterilizer for thirty minutes on three suc- 
cessive days. 
VI. Dextrose, Lactose, and Saccharose 
Bouillon. . 
(a) After filtration of the meat-infusion, prepared 
as above described, place in an Erlenmeyer flask, 
and inoculate with a fluid culture of the Bacillus 
coli or an allied gas-producer. 
(4) Place in the incubator at 37.5° for twenty- 
four hours. 
Fic. 31.—The Smith fer- Fic. 32.—A graduated fer- 
mentation-tube. mentation-tube. 
This removes the ineat-sugar. 
(c) Fiom this infusion prepare bouillon in the 
ordinary way. 
(2) To the completed broth add 1 per cent. of 
the required sugar. 
(e) Distribute in test-tubes or in fermentation- 
tubes,' and sterilize in the steam sterilizer on three 
successive days. 
1A small test-tube inverted inside a large one will 
answer for a fermentation-tube. 
