62 CULTURES OF BACTERIA. 
tube ; withdraw the needle carefully and replace 
the cotton plug. 
(2) Allow to growat the room-temperature, if gela- 
Fic. 37.—a,‘‘ Stab ”’ cult- 
ure; 5, ‘‘stroke’’ cult- 
ure. 
tin is used ; at either room- 
or incubator-temperature, 
if agar is used; examine 
from day to day. 
(3) Note in stab cult- 
ures: 
(a) Does the growth ap- 
pear along the line of 
puncture, at the surface, 
or in both places? 
(4) Is the surface growth 
abundant or scanty; does 
it spread over the whole 
surface or is it confined to 
the point of puncture ; is 
its margin regular or in- 
dented ; is it flat or raised, 
dry or moist; what is its 
color, luster ? 
(c) Is the medium 
changed in color, odor, 
or condition ? 
(2) In gelatin cultures, 
if liquefaction takes place, 
is it at the surface, deep, 
or throughout the line of 
puncture? What is the 
form of the liquefied area. 
The following terms have been suggested by 
