DEMONSTRATION OF CAPSULES. 33 
3. Boni’s Method.! 
(a) Prepare a solution as follows : 
White of one egg ; 
Glycerin, 50 '¢.c. 3 
Formalin, 2 drops. 
Shake together and filter. 
(6) Prepare films of the organism to be studied, 
using the above solution in place of water. 
(c) Spread very thin, and heat over flame until 
the glycerin is evaporated, 
(d@) Stain in Ziehl’s carbol-fuchsin’ for twenty 
to thirty seconds. 
(e) Wash in water and dry with filter-paper. 
(/) Contrast-stain in Léffler’s methylene-blue* 
for four to six minutes. 
- (g) Wash, dry, and mount in balsam. 
1 Centralbl. fiir Bakteriologie, Erste Ab., Band xxviii., 
No. 20, p. 705. 
2 See Appendix, page 172. 5 See Appendix, page 171. 
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