32 MORPHOLOGY OF BACTERIA. 
(2) Wash in anilin-water gentian-violet repeat- 
edly until all the acid is removed. 
(ec) Wash in a } per cent. solution of sodium 
chlorid and’examine in the same solution.. 
2. Johne’s Method. 
(a) Prepare films as usual. 
(6) Warm in a 2’per cent. solution of gentian- 
violet until steam arises. 
Fic. 16.—Bacterium pneumoniz of Friedlander, from the 
expectoration of a pneumonia patient; x 1000 (Frankel 
and Pfeiffer). 
(c) Wash in water. 
(@) Place in 2 per cent. acetic acid for six to ten 
seconds. 
(€) Wash in water. 
(/) Dry and mount in balsam. 
