STAINING FLAGELLA. 29 
(g) Wash in water, dry, mount, and examine 
with oil-immersion lens. 
4. Modification of Loffler’s Method. - 
(a) Prepare a mordant as follows : 
Tannic acid (20 per cent. solu- 
tion), TO €.6, 5 
Ferrous sulphate (saturated so- 
lution), 10 GC; 
Logwood solution (x gram 
boiled in 8 cc. of water 
and filtered), 3-4 CC. 
(4) Prepare a stain as follows : 
Anilin-water,' IOO C.C.; 
Sodium hydrate (1 per cent.), tee; 
Methylene-violet or methylene- 
blue or fuchsin, 4-5 gm. 
Filter. 
(c) Prepare films as before. 
(2) Heat with the mordant until steam rises, and 
then move over flame for one minute. 
(e) Wash in water, and if mordant does not dis- 
appear, in absolute alcohol. 
(/) Dry and heat with the stain until steam rises, 
then leave in the warm stain one minute. 
(g) Wash, dry, and mount. 
1 See Appendix, page 171. 
