28 MORPHOLOGY OF BACTERIA. 
Filter each time before using. This mordant will 
keep two or three days. 
(4) Prepare films as before. 
(¢) Dry and pass once through the flame. 
(@7) Cover the film with the mordant, and warm 
over the flame for a few seconds only. 
Fic. 14.—Bacillus typhosus, from an agar culture six 
hours old, showing the flagella stained by Léffler’s method; 
x 1000 (Frankel and Pfeiffer). 
(e) Wash in water, dip in absolute alcohol, and 
again wash in water. 
(f) Cover the film with anilin-water fuchsin,! 
and warm over the flame from three to four min- 
utes, taking care that the solution steams, but does 
not boil. 
1 See Appendix, page 171. 
