STAINING FLAGELLA. 27 
(4) Prepare solution B as follows : 
Tannic acid, I gm.; 
Distilled water, 19, ¢.c. 
(c) Filter when cold, mix the two solutions, and 
use immediately. 
(@) Prepare films as before. 
(e) Treat with the above mixture, gently heating 
until it almost boils, then set aside for a minute. 
(/) Wash, dry, and mount. 
Fic.13.—Microspira comma, showing the flagella; x 1000 
(Ginther). 
3. Loffler’s Method. 
(a) Prepare a mordant as follows : 
Tannic acid (20 per cent. solu- 
tion), filtered, IO C.c.3 
Ferrous sulphate (cold, satu- 
rated solution), filtered, ones 
Fuchsin (saturated alcoholic so- 
lution), 1 c.c. 
