EXAMINATION OF STAINED BACTERIA. 21 
(e) Wash in water. 
(/) Contrast-stain in an aqueous solution of 
eosin (1: 1000) for one-half to one minute. 
(g) Wash in 60 per cent. alcohol "for thirty 
seconds. 
(2) Wash in absolute alcohol for thirty seconds. 
(z) Clear in xylol and mount in balsam. 
(3) Third Method, 
(2) Stain in Kuhne’s methylene-blue! for one- 
half to one hour, or in carbol-thionin-blue? for five 
minutes. 
(4) Wash in water. 
(c) Treat with 0.5 per cent. acetic acid till pale 
green. 
(7) Wash in water, 60 per cent. alcohol, and 
absolute alcohol, each for thirty seconds. 
(e) Contrast-stain as in above methods, clear, and 
mount. 
(4) Fourth Method. 
(a) Stain the dried preparations in a dilute aque- 
ous solution of methylene-blue. 
(4) Wash in water and dry. 
(c) Stain in aqueous eosin solution (1 : 1000) for 
one to one and a half minutes. 
(2) Dehydrate, clear, and mount. 
1 See Appendix, page 171. 
2 See Appendix, page 171. 
