CONTENTS. 
CHAPTER I. 
_ 
Microscopic EXAMINATION OF BACTERIA .........4, II 
Manipulation of the Microscope, 11.—Measurement of Bac- 
teria, 11.—Examination of Living Bacteria, 13.—Ordinary 
Examination, 13.—Hanging Drop, 15.—Examination of 
Stained Bacteria, 16.—Ordinary Stains, 16.—Gram’s Stain, 
19.—Staining Bacteria in Tissues, 20. 
CHAPTER II. 
MorPHOLOGY OF BACTERIA . Ce ee ee 22 
Demonstration of Form, 22, Benenden of Motion, 24.— 
Staining Flagella, 25.—Demonstration of Capsules, 31. 
CHAPTER III. 
REPRODUCTION OF BACTERIA . = 2 ......4.4. 34 
Division, 34.—Spores, 35.—Staining Spores, 36.—Germina- 
tion of Spores, 38. 
CHAPTER IV. 
CLASSIFICATION OF BACTERIA .... «2... ses 39 
CHAPTER V. 
STERILIZATION © we te ee 43 
Steam, 46.—Autoclave, 46.—Hot-air, es 
CHAPTER VI. 
PREPARATION OF CULTURE-MEDIA .  ... ean 50 
Bouillon, 50.—Gelatin, 50.—Agar, 50.—Media from Meat- 
extracts, 55.—Potato, 55.—Dextrose, Lactose, and Saccharose 
Bouillon, 56.—Milk and Litmus Milk, 57.—Blood serum, 59. 
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