ANAEROBIC CULTURES. 79 
with pyrogallic acid made alkaline with sodium 
hydrate, and is sealed tightly with a rubber stopper. 
The inner tube contains the culture. The oxygen 
is absorbed by the mixture in the outer tube. 
3- Wright’s Method.—Make an ordinary cult- 
ure in a test-tube. Clip off any superfluous cotton 
from the plug, and push the plug into the tube so 
Fic. 49.—Novy’s jars for anaérobic cultures. 
that it lies 1 centimeter below the mouth. For 
test-tubes 6 X 34 inches, run into the cotton plug, 
from a pipet, approximately % c.c. of a freshly 
prepared solution of pyrogallic acid (1 part of acid, 
I part of water), and then approximately 1 c.c. of 
a solution of sodium hydrate (1 part of sodium 
hydrate, 2 parts of water). Quickly insert a rubber 
stopper into the tube. 
4. Make a hanging-drop culture. On one side 
of the cover-glass introduce a little pyrogallic acid, 
and on the other side a little sodium hydrate, so 
that it runs around and unites with the acid. Seal 
with vaselin, 
