80 CULTURES OF BACTERIA. 
5. Distribute the germs to be studied in bouillon 
or in liquefied gelatin or agar, and draw some of 
the solution into sterile pieces of glass tubing of 
small caliber. When the tube is full seal the ends 
in a flame. 
6. Put large quantities of culture-medium in the 
tubes and puncture deeply. The surface of the 
medium is then covered with sterile oil. 
7. Park’s Method.—Cover the culture-medium 
with melted paraffin.’ Sterilize by the ordinary 
methods, and when cool enough for inoculation, 
but before the paraffin solidifies, inoculate through 
the paraffin into the medium below. 
Wright recommends? the following precautions 
in growing anaérobic bacteria : 
1. ‘The medium should contain 1 per cent. of glu- 
cose, and should be boiled and cooled immediately 
before inoculation. 
2. The medium should be freshly prepared. 
3. The reaction should not be more acid to 
phenolphthalein than +1.5. With 1 per cent. 
glucose bouillon, growth is better if the reaction is 
nearer the neutral point of phenolphthalein than 
+15. 
VHI. DEMONSTRATION OF LIQUEFYING FERMENT. 
(a) Inoculate several gelatin-tubes with Bacellus 
prodigiosus. 
(4) Allow them to grow until all the gelatin is 
liquefied. 
1 Melting point, 42° C. 
2 Jour. Boston. Soc. Med. Sci., vol. v., No. 4, p. 114. 
