STANDARD CHART FOR BACTERIAL ANALYSIS. 
INTAWME cance: cesecsccssnscseacth POUR CE cccre: steers asus HaBITAT.. TAI i execencisdoncehdcrnases REPORTED BY wrcsccccescccsssctsenet 
Form and arrangement in bouillon, grown ............. hours at 18°-20° C.; ditto, grown ............. hours at 36°-38° C. 
Size, length ............. Hy breadth ............- #; extreme lengths from ............. PERO recsccreier: B. 
Capsules, none observed, easily observed or demonstrated. Conditions under which they are present, agar, serum, milk, or 
Spores, none observed within ............. hours at 
Germinate within ............. hours at ... 
When present are polar, central, cells ............. swollen. 
°C. Stain by .method. Are killed at 100° C. in .. 
.° C., or when stained with 2.000.000... 
Vacuoles, observed when grown on 
Motility, sluggish or active, rotary or direct, more pronounced in -.......-e cultures grown at ............ OCS FOr cassie hours. 
Flagella stain by ......................... method ; are monotrichous, lophotrichous, amphitrichous, peritrichous. ~- 
Pleomorphism, observed in 0.2.1.0: sees cultures grown at ............. ONG AOR cccacees days. 
Stain, easily or with difficulty with ..........-...+ , uniformly or irregularly. Stained or decolorized by Gram’s method. 
Gainers Boar Surface colonies. Deep colonies. BouiLion. SkeTcH of Germ AND COLony. 
PLaTEs. = é Opacity begins after .... hrs. at ....°C. 
si cane Gelatin. Agar. Gelatin. Agar. Pellicle forms in .... hrs. at ....° C. 
| os Color .... appears in’... hrs. at ....°C. 
ae —, 
i Deposit forms in .... hrs. at ....9 C. 
Under mica plate. ma Stas CULTURES. 
; ; Character, compact, flocculent, granular, 
GELATIN OR AGAR-TUBE. flaky, or viscid on agitation. 
Puncture. Color. é 
Form Odor. 
. rT °° 
| Surareipiowels « .* Reaction, wee sahesnat joa Ce 
Size. 
Shape. 
pone Potato or BLoop-sERuM. 
Color. | ee 
Consistence. M ape 
Deep growth. Surce veliee 
anes Laas ap 4 St Se te =e 3 J g fs ve - : eeat re 
ee SHEE bbles. ae ee ne ats cn PES RCULTURESS OT 
Size. Texture. » <= ss Pa aaa 
Shape. Consistence, 1S) iS) 
Margin. Liquefaction. ° 3 
Surface relief. Gas production. : : 
Light transmission. fe 
Color. 4 4 
Luster. a 
Consistence. os aa . 3 : 
: ; eduction of nitrates. 2 
— in medium. Dyers ices bouillon. Pa - 
‘olor. ndol production. é A 
Consistence. ¥ > YZ : eZ 
Odor. 
MILK. 
Curd, curdles or does not curdle in .... days at 18°-20° C. or aes Ler 
ee Ge eS en ‘boiling after .... daysat ....°C. 
hard or soft, in one mass or in fragments, gas-bubbles. 
changed or unchanged by boiling. 
W hey, separates from curd or not, amount, transparent or turbid. 
Reaction, w..e.206 AD: scacaaasa days at. 32 fey Color. Gas production. 
Digestion becomes gradually transparent without forming curds. 
complete in .... daysat. 
effects of boiling, odor, clear or cloudy, watery or viscid. 
reaction at end of digestion sievenicravonaeiae 
2S Saas i a : = apeere z aoe ae 
Sucar BovuiLion IN ~ Amount of gas in ae at Reaction of fluid in. A Pelli- | Opac- Col 
FERMENTATION-TUBES 18°-20° C, 60-389 C. after....days at. h cle. ity. OOK: 
Micrococcus, single, pairs, chains, tetrads, or cubical packets ; Bacil/us, single, pairs, chains, or filaments; Spzrzd/m, comma, spiral. 
inzd. as BS iy eT inrd,, 2ds. a tegen ENR 
DDOREHOSE eacigesicsl| ides Sees WES OAH MERE | YE FT Seg GEES VT 
EpCtosecinscmdscee | Goan, satus Sens HPS KEN || Re Soe Skee SHES Gees 
Saccharose.. #3 
PIGMENT, PATHOGENESIS. 
developed in presence or absence of oxygen. 
es TE, onensemant cultures at ...... °C.in hours. 
color 5 by acid or alkali. 
soluble in 
Optimum Temperature ....0C. Growrn Limits ....°C.to....°C. THermat Dearnu-point ....°C., time of exposure .... minutes. 
PropucTion oF Acips oR ALKALIES. RELATION TO FREE OXYGEN. RetaTion oF GrowTH To AcipiTy or ALKALINITY OF MEDIUM. 
Carbohydrates absent or present. Obligatory aérobe. -... @acid to.... % alkaline. 
Facultative anaérobe. 
Obligatory anaérobe. 
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