PYOGENIC ORGANISMS. 125 
may by accident be spilled on floor or table. Care- 
fully sterilize everything that the pathogenic 
material may have contaminated. Thoroughly dis- 
infect the hands, instruments, and table with the 
corrosive sublimate solution after completing the 
work. 
Fic. 58.—Streptococcus pyogenes (from a bouillon culture). 
I. PYOGENIC ORGANISMS. 
(a) Study, according to the schedule in Chapter 
VIII., the morphology and biology of : 
Micrococcus aureus (Staphylococcus pyogenes aureus). 
Micrococcus citreus (Staphylococcus pyogenes citreus). 
Micrococcus pyogenes (Staphylococcus pyogenes albus). 
Streptococcus pyogenes. 
Sarcina tetragena (Micrococcus tetragenus). 
(4) Select and weigh a well-grown rabbit. In- 
oculate into the ear vein by means of a hypodermic 
syringe’ I cc. of a twenty-four or forty-eight hour 
bouillon culture of one of the above species, or of 
pus taken directly from an abscess. 
1 Keep the syringe for some time before the operation in 
a 2 per cent. solution of carbolic acid. Wash it out five or 
six times with sterile water or bouillon before the inocula- 
tion; or the syringe and needle may be boiled for five 
minutes before using. 
