PYOGENIC ORGANISMS. 127 
(e) Preserve some of tissues in absolute alcohol 
or in Zenker’s fluid.! 
(/) Stain the smear preparations and examine 
for the inoculated organism. 
(g) Make pure cultures from whatever growth is 
Fic. 61.—Streptococcus pyogenes ; cover-glass preparation 
from the pus of an abscess ; x 1000 (Frankel and Pfeiffer), 
obtained in the cultures from the blood and organs, 
and try to recover the original organism. 
them ; sterilize the blades of the knives, scissors, and for- 
ceps used in making the incisions by dipping them in 
methyl alcohol and passing them quickly near enough to a 
Bunsen flame to ignite the alcohol. Smears and cultures 
from the blood are best made from the blood in the heart. 
For the method of preparation of blood-smears, see page 
59. 
1 See Appendix, page 173. 
