GONOCOCCUS. 129 
(Z) Section the preserved tissue and stain for 
bacteria with carbol-thionin-blue, Kuhne’s methy- 
lene-blue, or by Gram’s inethod. 
Fic. 64.—Mouse-holder, with mouse in position for inocu- 
lation. 
Other animals—guinea-pigs, rats, or mice—may 
be used for inoculations. Inoculations may be sub- 
cutaneous, peritoneal, or intravenous, according to 
the organisms used or the nature of the experi- 
ment. 
Il. GONOCOCCUS. 
1. Examine cultures of Micrococcus gonorrhee, 
and stain the organism with Loffler’s blue and by 
Gram’s méthod. 
2. Examine gonorrheal pus as follows : 
(a) Prepare films on cover-glasses. 
(4) Pass three times through flame. 
(c) Stain with Léffler’s methylene-blue or with 
any aqueous anilin stain one minute. 
(d) Wash, dry, and mount. 
(e) Examine with the oil-immersion lens. 
