GLANDERS, 133 
IV. GLANDERS. 
1. Study the morphology and biology of the 
glanders bacterium. 
2. Inoculate a male guinea-pig intraperitoneally 
with 1 c.c. of a bouillon culture. 
3. Note in two or three days the great swelling 
Fic. 68.—Bacterium anthracis, stained to show spores; 
x 1000 (Frinkel and Pfeiffer). 
and redness of the testicles, caused by a semipuru- 
lent affection of the tunica vaginalis. This is a 
diagnostic test for the glanders bacterium. 
4. At the autopsy prepare smears, cultures, and 
sections from all the organs and from the peritoneal 
and scrotal nodules. 
5. Stain sections as follows : 
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