134 PATHOGENIC BACTERIA. 
(2) Carbol-thionin-blue for ten to fifteen minutes. 
(4) Wash thoroughly in water. 
(c) Dehydrate in anilin oil. 
(2) Treat with equal parts of anilin oil and xylol. 
(e) Pass through pure xylol to balsam. 
Fic. 69.—Bacterium mallei, from a culture upon glycerin 
agar; x 1000 (Frankel and Pfeiffer). 
6. For the diagnosis of a suspected case of glan- 
ders proceed as follows : 
(a) Rub a large swab made of absorbent cotton 
in the discharge from the nose or in the suspected 
ulcer. 
(4) Transfer the swab to 5 c.c. of sterile water 
and shake thoroughly. 
(c) Inoculate the resulting suspension intraperi- 
toneally into a well-grown male guinea-pig. 
