DIPHTHERIA, 135 
(7) In two to seven days scrotal inflammation 
will develop if the glanders bacterium was present. 
If the glanders bacterium was not present, after a few 
hours or days of depression the guinea-pig recovers 
completely. It may happen that some acute septic 
organisms were present in the material injected. 
In such cases the guinea-pig usually dies within 
twenty-four hours, and the test is evidently of no 
value and must be repeated. 
(e) If the scrotal lesions appear, perform an 
autopsy. Look particularly for. nodular deposits in 
the peritoneum and visceral layer of the tunica 
vaginalis, infiltration of the scrotal tissue, and 
edema extending into the groin and suprapubic 
region. 
(f) Transfer aseptically a portion of a nodule to 
potato, and place at 37.5° C. In twenty-four to 
forty-eight hours siall, smooth, glistening, amber- 
colored colonies of the glanders bacterium should 
develop. The bacterium is a short, thick rod, with 
rounded ends, usually slightly curved or bent, 
soinetimes elongated into threads. It stains faintly 
in Loffler’s methylene-blue. 
(g) A positive diagnosis can usually be made 
from the gross lesions, but the isolation of the 
organism makes the diagnosis certain. 
Vv. DIPHTHERIA. 
1. Make swabbings from the throats of healthy 
individuals and from several diphtheria patients in 
hospital. Depress the tongue and rub a sterile 
