140 PATHOGENIC BACTERIA. 
(a) Prepare a twenty-four to forty-eight hour 
bouillon culture. 
(4) Sterilize a hypodermic syringe and needle by 
soaking in 2 per cent. carbolic acid and washing 
out in sterile water or bouillon, or by boiling for 
five minutes. 
(c) Select and weigh a full-grown guinea-pig. 
(d) While the pig is held on its back on the table 
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Fic. 74.—Slide, 7 x 2} inches, for the routine examina- 
tion of diphtheria cultures. Each square can be placed 
under the lens of the microscope without disturbing the 
equilibrium of the slide. 
by an assistant, the hair is removed from a small 
area on the ventral abdominal wall. 
(e) With the thumb and forefinger of the left 
hand pinch up a fold of the skin, and with the 
right hand insert the hypodermic needle between 
the skin and the muscular body-wall. 
(/) Inoculate an amount of the culture equal to 
I per cent. of the weight of the pig. 
(g) Record the daily weight of the pig until 
death. 
