TUBERCULOSIS. 53 
particles, place between two cover-glasses, and 
spread evenly. 
(c) Draw the covers apart and allow the films to 
dry. 
‘(d) Pass three times through flame. 
ayy 
¢ 
J 
Fic. 88.—Tubercle bacteria in sputum (carbol-fuchsin and 
methylene-blue). 
(e) Stain in hot (steaming but not boiling) 
Ziehl’s carbol-fuchsin for five minutes or in the 
cold solution for twenty minutes. 
(7) Wash rapidly in water. 
(g) Decolorize in 20 per cent. sulphuric, hydro- 
chloric, or nitric acid, for three to five seconds, 
