TUBERCULOSIS. 155 
in sweet spirits of nitre to which has been added 
enough alcoholic solution of malachite-green, Bis- 
marck-brown, or methiylene-blue to give a deep- 
colored fluid. 
(c) Wash in water, dry, and mount. 
3. Inoculations. 
(a) Inoculate a guinea-pig subcutaneously in the 
abdoniinal wall with tuberculous material. 
Fic. 89.—Tubercle bacteria in sputum (Frankel and 
Pfeiffer). 
(6) After four to six weeks,' when the inguinal 
lymphatic glands have become enlarged, kill the 
animal, 
(c) With proper aseptic precautions make three 
or four cultures on blood-serum from two or three 
1 For class-work such an animal must be previously inoc- 
ulated. 
